ISSN No.
2395-3306
(Online)
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 Volume  4 / Issue 1 / September 2016   (pp 1-14)
Screening of Phage Display Recombinant Antibodies against Nucleoprotein antigen of Avian Influenza from Periplasmic Extract and Media supernatant of cultured TG1 and HB2151 cells Devyani Sharma, Sandhya Rani Panda* and Ashish Saraf
Int. J. Chem. Pharm. Rev. Res., 2016, 4(1), pp 1-4  •  Abstract  •  Fulltext    •   Citations: 0    •   Article Views: 0
 Nucleoprotein gene from Avian Influenza virus was cloned in Phagemid and expressed in prokaryotic system. E.Coli TG1 cells were chosen according to the specificity of the Phagemid Vector.  pCANTAB 5E, Phagemid Vector is able to infect an array of strains of E. Coli cells. TG1 cells can produce both phage display antibodies as well as soluble antibodies. The secreted antibodies from both the sources i.e. from Periplasmic space and the supernatant, and were screened using ELISA with the specific antigen and it was found to have higher yield in the Periplasmic space as compared to media supernatant. These clones showing higher yield in the Periplasmic space were selected to be cloned in Expression vector E.Coli HB2151. The expression, as seen in western blotting was found to increase manifold. These cells did not show presence of antibodies in supernatant. 
A Comparative Database Reflecting Variation in Intelligence Quotient (IQ) of Children of Central Odisha with fluoride concentration Deepa Nag & Aparna Dutta*
Int. J. Chem. Pharm. Rev. Res., 2016, 4(1), pp 5-8  •  Abstract  •  Fulltext    •   Citations: 0    •   Article Views: 0
 Exposure to geo-chemical toxins, such as excess fluoride is one of the important factors for the variation of IQ of children. Fluoride ions mostly enroute the human body through drinking water. The present investigation centers around quantifying the effect of fluoride poisoning on the intellectual development of children residing in villages of central Odisha who use ground water for drinking purpose. For this purpose a comparative study was made between IQ level of children exposed to different fluoride concentration. The area selected for investigation were villages of Nayagarh district having F- >3mg/L and villages of Boudh district having F-≤1.5mg/L. IQ level of children was measured by using Raven’s Standard Progressive Matreces Test . Data were statistically  analysed by z- test with a significant level of p = 0.05. The mean IQ scores decreased from 107±16 for normal fluoride group to 88±15 for high fluoride group. Also the average IQ level of normal and children suffering from dental fluorosis in the regions having low and high F- concentration are compared. The average IQ of dental fluorosis sufferers is clearly lower than that of normal children and this difference is statistically significant.

Ameliorative Effect of Momordica Charantia against Arsenic Induced Hepatotoxicity in Albino Mice R.Maheswari1*, S.Divya2, S.Suresh Kumar3, B. Senthil Kumar4 and S.Arivoli5
Int. J. Chem. Pharm. Rev. Res., 2016, 4(1), pp 9-14  •  Abstract  •  Fulltext    •   Citations: 0    •   Article Views: 0
 In our present study, an attempt has been made to study the effect of Arsenic trioxide on biochemical parameters and ameliorating effects of the aqueous fruit (vegetable) extract of Momordica charantia L (MC), Karela, (Bitter Melon) in swiss albino mice. The aim and purpose of our study was to evaluate the ability of aqueous extracts from the whole fruit Momordica charantia L to protect normal hepatocytes against oxidative damage in-vitro. The present study was conducted to evaluate the protective role of Momordica against arsenic induced hepatotoxicity in albino mice. Albino mice were divided into three groups. Our results imply that Momordica charantia L has the protective antioxidant properties. Group I were control mice, Group II received an acute dose of arsenic (5 mg/kg bw) orally, Group III received an acute dose of arsenic followed by daily administration of Momordica (20mg/kg bw) orally. Autopsies were done on 15 days post treatment. Arsenic trioxide treatment leads to increase in weight of liver. Biochemical analysis of treated group showed decrease in antioxidant enzymes, viz., SOD, CAT but increased MDA content in liver as compared to control group. MC administration to mice decreased the weight of mice and showed significant protection in the alleviation of arsenic induced hepatic injury.

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